Journal: iScience

Article Title: Correlative multiscale 3D imaging of mouse primary and metastatic tumors by sequential light sheet and confocal fluorescence microscopy

doi: 10.1016/j.isci.2025.111934

Figure Lengend Snippet: Overview of the procedure and average times required for each step

Article Snippet: 11 , 3D image processing & registration , Varies , • Construct the correlated multiscale 3D image using Fiji and Imaris software • Segment and analyze the image data using Fiji software.

Techniques: Solvent, Agarose Gel Electrophoresis, Fluorescence, Microscopy, Software, Imaging, Incubation, Sequencing, Immunofluorescence, Staining, Confocal Laser Scanning Microscopy, Multiplex Assay, Construct

Journal: iScience

Article Title: Correlative multiscale 3D imaging of mouse primary and metastatic tumors by sequential light sheet and confocal fluorescence microscopy

doi: 10.1016/j.isci.2025.111934

Figure Lengend Snippet: Correlative multiscale 3D imaging to localize a metastatic lesion in a whole mouse brain (A) 3D rendering and virtual slicing of the whole mouse brain images acquired by 3D LSFM scanning of autofluorescence signal. Scale bar: 3 mm. (B) Virtual cross-section view featuring a ROI. A yellow dotted box indicates the ROI. (C) 3D rendering of the whole brain macrosection image acquired by CLSM. The brain macrosection was stained for DAPI+ (blue) cell nuclei, CK8+ (orange) tumor, CD45 + (yellow) immune cells, and ER-TR7+ (green) fibroblasts. A red dotted box presents the ROI. Scale bar: 3 mm. (D) 3D rendering of the ROI obtained by high-resolution CLSM. This cellular resolution 3D image shows the metastatic lesion of CK8+ mouse mammary cancer cells (orange) and the response of CD45 + immune cells (yellow) to the cancer invasion. Scale bar: 50 μm.

Article Snippet: 11 , 3D image processing & registration , Varies , • Construct the correlated multiscale 3D image using Fiji and Imaris software • Segment and analyze the image data using Fiji software.

Techniques: Imaging, Staining

Journal: Nature

Article Title: Clonal hematopoiesis and risk of chronic liver disease

doi: 10.1038/s41586-023-05857-4

Figure Lengend Snippet: a, Prevalence of liver inflammation and hepatic steatosis on magnetic resonance imaging among 8,251 individuals in the UK Biobank. Liver inflammation and fibrosis was defined as a cT1 signal ≥ 795 ms. Fatty liver was defined as a proton density fat fraction ≥ 5%. Logistic regression, with adjustment for age and sex, was used to test the association between CHIP status and the presence of fatty liver and liver inflammation. b, Perspectum MultiScan cT1 image (left) and proton density fat fraction (right) of a patient with CHIP. Image reproduced with permission from the UK Biobank. c–g, B6.SJL mice were transplanted with Tet2−/− (n = 20), Tet2−/−Nlrp3−/− (n = 10) or control vavCre+ (wild type (WT); n = 13) bone marrow cells and fed CDAHFD for 11 weeks. Steatohepatitis was graded using modified NASH CRN (Clinical Research Network) histologic criteria. Compared to vavCre+ and Tet2−/−Nlrp3−/− animals, Tet2−/−-transplanted mice show similar accumulation of liver fat (c), increased inflammation (d), macrophage crown structures (e, arrows) and hepatocyte ballooning (f, arrowhead), resulting in a higher aggregate NAS (g). h, B6.SJL mice were transplanted with Tet2−/− (n = 24) or vavCre+ wild-type control (n = 21) bone marrow cells and fed CDAHFD for 19 weeks. Collagen fibrosis, highlighted by Picrosirius red staining (left and middle panels), was quantified as the percentage of positive area using ImageJ (right panel). Statistical analysis was performed using two-tailed unpaired t-test (a), chi-square test (g) and Mann–Whitney test (h). For a and h, error bars represent mean ± s.d.

Article Snippet: Logistic regression, with adjustment for age and sex, was used to test the association between CHIP status and the presence of fatty liver and liver inflammation. b , Perspectum MultiScan cT1 image (left) and proton density fat fraction (right) of a patient with CHIP.

Techniques: Magnetic Resonance Imaging, Control, Modification, Staining, Two Tailed Test, MANN-WHITNEY